Accession | CRX032436 |
Organism | Strongylocentrotus purpuratus |
Title | sea urchin sperm methylome rep1 |
BioProject | PRJCA000098 |
BioSample | SAMC047984 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA spiked with 0.5? unmethylated Lambda DNA (Promega) was sonicated into 100-500bp. Fragmented DNA was then subjected to end repair,dA tailing, ligation with the end repair enzyme mix (NEB), Klenow 3´-5´ exo- (NEB) and T4 DNA ligase (NEB), respectively. Adapter-ligated DNA was recovered by 2? agarose gel electrophoresis, and bisulfite conversion was performed using the EZ DNA methylation-Gold kit (Zymo Research) according to the instruction manual. Bisulfite-treated DNA was amplified with KAPA HiFi HotStart Uracil+ ReadyMix. Amplified DNA was recovered by 2? agarose gel electrophoresis. |
Bisulfite-Seq |
GENOMIC |
RANDOM |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 300
|
Release date | 2019-05-30 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR036572 |
CRR036572_f1.fastq.gz
CRR036572_r2.fastq.gz
|
4,286.12
3,964.52
|
CRR036573 |
CRR036573_f1.fastq.gz
CRR036573_r2.fastq.gz
|
9,324.71
8,851.97
|
CRR036574 |
CRR036574_f1.fastq.gz
CRR036574_r2.fastq.gz
|
10,075.74
9,603.17
|
|
Submitter | xiaocui xu (xuxc@big.ac.cn) |
Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
Date submitted | 2018-11-13 |