| Accession | CRX032438 |
| Organism | Ciona savignyi |
| Title | ciona gastrula methylome rep1 |
| BioProject | PRJCA000098 |
| BioSample | SAMC047986 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA spiked with 0.5? unmethylated Lambda DNA (Promega) was sonicated into 100-500bp. Fragmented DNA was then subjected to end repair,dA tailing, ligation with the end repair enzyme mix (NEB), Klenow 3´-5´ exo- (NEB) and T4 DNA ligase (NEB), respectively. Adapter-ligated DNA was recovered by 2? agarose gel electrophoresis, and bisulfite conversion was performed using the EZ DNA methylation-Gold kit (Zymo Research) according to the instruction manual. Bisulfite-treated DNA was amplified with KAPA HiFi HotStart Uracil+ ReadyMix. Amplified DNA was recovered by 2? agarose gel electrophoresis. |
Bisulfite-Seq |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 300
|
| Release date | 2019-05-30 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR036576 |
CRR036576_f1.fastq.gz
CRR036576_r2.fastq.gz
|
3,341.86
3,142.83
|
| CRR036577 |
CRR036577_f1.fastq.gz
CRR036577_r2.fastq.gz
|
11,169.29
10,769.69
|
|
| Submitter | xiaocui xu (xuxc@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-11-13 |
|---|
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