| Accession | CRX032449 |
| Organism | Mus musculus |
| Title | mouse placenta methylome |
| BioProject | PRJCA000098 |
| BioSample | SAMC047997 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA spiked with 0.5? unmethylated Lambda DNA (Promega) was sonicated into 100-500bp. Fragmented DNA was then subjected to end repair,dA tailing, ligation with the end repair enzyme mix (NEB), Klenow 3´-5´ exo- (NEB) and T4 DNA ligase (NEB), respectively. Adapter-ligated DNA was recovered by 2? agarose gel electrophoresis, and bisulfite conversion was performed using the EZ DNA methylation-Gold kit (Zymo Research) according to the instruction manual. Bisulfite-treated DNA was amplified with KAPA HiFi HotStart Uracil+ ReadyMix. Amplified DNA was recovered by 2? agarose gel electrophoresis. |
Bisulfite-Seq |
GENOMIC |
RANDOM |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 300
|
| Release date | 2019-05-30 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR036599 |
CRR036599_f1.fq.gz
CRR036599_r2.fq.gz
|
4,919.07
4,730.34
|
| CRR036600 |
CRR036600_f1.fq.gz
CRR036600_r2.fq.gz
|
5,392.63
5,207.81
|
| CRR036601 |
CRR036601_f1.fq.gz
CRR036601_r2.fq.gz
|
5,142.94
4,967.75
|
| CRR036602 |
CRR036602_f1.fq.gz
CRR036602_r2.fq.gz
|
4,335.12
4,100.63
|
| CRR036603 |
CRR036603_f1.fq.gz
CRR036603_r2.fq.gz
|
4,644.1
4,369.43
|
| CRR036604 |
CRR036604_f1.fq.gz
CRR036604_r2.fq.gz
|
4,004.76
3,799.18
|
|
| Submitter | xiaocui xu (xuxc@big.ac.cn) |
|---|
| Organization | Beijing Institute of Genomics, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-11-13 |
|---|
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