实验基本信息
实验编号 CRX038448
物种名称 Plutella xylostella
标题 mix_above3k
项目编号 PRJCA001194
样本编号 SAMC053466
测序平台 PacBio RS II
建库信息
文库名称 文库构建方法 建库策略 样品来源 实验选择 文库布局
Total RNA of the 10 samples were mixed together in equal parts (2 µg for each sample). The total RNA was reverse transcribed using the Clontech SMARTerTM PCR cDNA synthesis Kit with anchored oligo(dT)30 as the primer. The double-strand cDNA was then amplified with LD-PCR (Long-Distance PCR) using Advantage 2 PCR Kit. We used the BluePinppin size selection system (Sage Science, Beverly, MA) to generate cDNA fractions with different sizes including 0.5-1Kb, 1-2 Kb, 2-3 Kb and >3 Kb. These libraries were then constructed with the Pacific Biosciences’ SMRTbell Template Prep Kit 1.0, following the manufacturer’s protocol. A total of six SMRT cells (2 SMRT cell for libraries of 1-2 Kb; 2 SMRT cell for libraries of > 3Kb; one SMRT cell for libraries of 0.5-1 Kb, and; one SMRT cell for libraries of 2-6 Kb) were sequenced on the PacBio RS II platform. FL-cDNA TRANSCRIPTOMIC unspecified FRAGMENT
处理信息
发布日期2019-01-20
测序反应
Run编号 发布日期 Run序列文件信息
File nameFile size (MB)
CRR043121 2019-01-20 above3k-2.tar.gz 20,322.63
提交lin jing (lolyemily@163.com)
所属单位Beijing Institute of Genomics, Chinese Academy of Sciences
提交日期2019-01-10