Experiment information
Accession CRX040556
Organism Scophthalmus maximus
Title investigate the influence of cecropin AD on intestinal microbiota of juvenile turbot, Scophthalmus maximus L.
BioProject PRJCA001244
BioSample SAMC056801
Platform Ion Torrent Proton
Library
Library name Construction protocol Strategy Source Selection Layout
The bacterial DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to previous reportSequencing libraries were constructed using the TruSeq Nano DNA LT Library Prep Kit. Sequencing of 16s rRNA V4 region was conducted on an S5 XL platform provided by Beijing Novogene Genomics Technology Co. Ltd. (Tianjin, China). A fast computational software-FLASH was used to merge Paired-end reads from the original DNA fragments into raw tags. Sequences were then analyzed using the QIIME (Quantitative Insights Into Microbial Ecology) software package (v1.7.0); The tags were then clustered to operational taxonomic units (OTUs) based on 97% sequence similarity cut off value OTHER GENOMIC unspecified FRAGMENT
Processing
Release date2019-02-18
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR045285 2019-02-18 CAD5.1.1.fastq.gz 13.33
CRR045286 2019-02-18 CAD5.2.1.fastq.gz 12.19
CRR045287 2019-02-18 CAD5.3.1.fastq.gz 13.77
SubmitterDai ji hong (15610059290@163.com)
OrganizationsOcean University of China.
Date submitted2019-01-25
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