| Accession | CRX045525 |
| Organism | Homo sapiens |
| Title | Huh7_SP2_HiC |
| BioProject | PRJCA001245 |
| BioSample | SAMC065117 |
| Platform | Illumina NovaSeq 5000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The Hi-C experiment was performed follow the in-situ Hi-C protocol. Briefly, cells were lysis and chromatin was digested by MobI, then biotin fill-in and Hi-C ligation. After ligation, DNA was purified and sonicated to 200-500bp for library construction follow the . DNA was end-repaired, dATP attached and adapter ligated follow the in-situ Hi-C protocl. After adapter ligation DNA was PCR amplified and library fragments between 200-500bp were purified for sequencing. The purified library was sequenced on NovaSeq at BerryGenomics Co., LTD. |
Hi-C |
GENOMIC |
Restriction Digest |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2019-10-01 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR050458 |
CRR050458_f1.fq.gz
CRR050458_r2.fq.gz
|
21,950.91
23,467.65
|
|
| Submitter | Xiaoyuan Song (songxy5@ustc.edu.cn) |
|---|
| Organization | University of Science and Technology of China |
|---|
| Date submitted | 2019-03-22 |
|---|
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