Experiment information
Accession CRX041687
Organism Pyrus ussuriensis
Title P. ussuriensis 'Huagai'
BioProject PRJCA001284
BioSample SAMC058635
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
High-quality genomic DNA was randomly interrupted by ultrasound. DNA fragments ranging from 150~800 bp were recovered by electrophoresis. T4 DNA Polymerase, Klenow DNA Polymerase and T4 PNK were used to repair cohesive ends to blunt ends. The 30 end of DNA fragment was amended including an “A” base and ligated to adaptors, including a 50 end T base. All fragments were recovered by electrophoresis and then paired-end sequenced by an Illumina HiSeq 4000. WGA GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 500
Release date2019-03-14
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR046441 2019-03-14 HGAI_R1.fastq.gz
HGAI_R2.fastq.gz
2,887.58
2,986.68
SubmitterShuang Jiang (271339616@qq.com)
OrganizationsShanghai Academy of Agricultural Sciences
Date submitted2019-02-19
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