Experiment information
Accession CRX041773
Organism Camellia sinensis
Title GBS:Tea 74
BioProject PRJCA001272
BioSample SAMC058767
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
Total genomic DNA of each tea plant sample was extracted from fresh leaves using plant genomic DNA rapid extraction kit (Biomed Gene Technology). The integrity, purity, and concentration of the extracted DNA were determined by 1% agarose gel electrophoresis and a Qubit Fluorometer (Invitrogen). One-hundred nanogram genomic DNA was digested with restriction enzymes SacI and MseI. The resulting samples were ligated with the restriction fragment using the designed sequence linker. The PCR ampli?cations were used to enrich the adapter-ligated DNA fragments. The inserted fragments with a length of 370-420bp were selected. Finally, the selected DNA fragments were enriched, amplified and sequenced using the Illumina HiSeq X ten platform basing on the Paired-End 140 (PE140) strategy. OTHER GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 140
Planned read length (bp) for mate 2: 140
Insert size (bp): 395
Release date2019-07-31
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR046527 2019-07-31 187_R1.fq.gz
187_R2.fq.gz
230.17
265.05
SubmitterSuZhen Niu (niusuzhen@163.com)
OrganizationsCollege of Tea Science, Guizhou University
Date submitted2019-02-22
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