Accession | CRX044017 |
Organism | Microbiota |
Title | 16S amplicon sequencing of plant root microbiome sampleThasKO1r17 |
BioProject | PRJCA001296 |
BioSample | SAMC063496 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA for each sample was extracted with FastDNA SPIN Kit (MP Biomedicals), then V5-V7(799F-1193R) of 16S rDNA was amplified by two-step PCR. Finally, the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 450
|
Release date | 2019-02-28 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR048806 |
CRR048806_f1.fq.gz
CRR048806_r2.fq.gz
|
15.81
17.79
|
|
Submitter | Yong-Xin Liu (woodcorpse@163.com) |
Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
Date submitted | 2019-02-27 |