Foc1 60 genome sequencing |
DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method, 20-kb inserts were constructed using the SMRTbellTM Template Prep Kit 1.0.Then was mechanically sheared by Covaris g-TUBE (Covaris, Woburn, MA, USA), followed by DNA damage repair and end-repair before ligating the hairpin adaptors to generate the SMRTbell template. A Blue Pippin device (Safe Science Inc., Beverly, MA, USA) was used for size selection with a cut-off value of 9,000 bp, and the size-selected library was purified using AMPure PB beads (Beckman Coulter, High Wycombe, UK).Then it was sequenced on PacBio Sequel system. |
WGS |
GENOMIC |
unspecified |
SINGLE
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