Experiment information
Accession CRX044425
Organism Fusarium oxysporum f. sp. cubense
Title Whole-genome sequencing of FocTR4 58
BioProject PRJCA001282
BioSample SAMC064005
Platform PacBio Sequel
Library
Library name Construction protocol Strategy Source Selection Layout
FocTR4 58 genome sequencing DNA was extracted using the cetyltrimethylammonium bromide (CTAB) method, 20-kb inserts were constructed using the SMRTbellTM Template Prep Kit 1.0.Then was mechanically sheared by Covaris g-TUBE (Covaris, Woburn, MA, USA), followed by DNA damage repair and end-repair before ligating the hairpin adaptors to generate the SMRTbell template. A Blue Pippin device (Safe Science Inc., Beverly, MA, USA) was used for size selection with a cut-off value of 9,000 bp, and the size-selected library was purified using AMPure PB beads (Beckman Coulter, High Wycombe, UK).Then it was sequenced on PacBio Sequel system. WGS GENOMIC unspecified FRAGMENT
Processing
Release date2019-04-28
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR049239 2019-04-28 Foc4_58.subreads.tar.gz 13,002.96
SubmitterLiangsheng Zhang (fafuzhang@163.com)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2019-03-15
Related experiments
Experiments(1)