| Accession | CRX048523 |
| Organism | Ovis aries |
| Title | we sequence this Ujimqin sheep WZS1 with whole-genome resequencing |
| BioProject | PRJCA001227 |
| BioSample | SAMC067963 |
| Platform | Illumina HiSeq 2000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Genomic DNA was extracted from ear tissue using the standard phenol-chloroform protocol. High-quality DNA for genome sequencing was processed to construct short-insert (500 bp) DNA libraries according to the manufacturer’s specifications. To generate 500-bp mate-paired libraries, we used the Covaris Ultrasonic Processor to cut genomic DNA into 500-bp fragments randomly, followed by the process of end repairing, adding A to the tails, purification and PCR amplification. The qualified libraries with appropriate insert size (500 bp) and concentration (> 2 nM) were sequenced using the Illumina HiSeq 2000 platform, and 100-bp paired-end reads were generated and managed using Illumina HiSeq Control Software (HCS) v3.3. |
WGS |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 500
|
| Release date | 2019-04-25 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR053686 |
CRR053686_f1.fq.gz
CRR053686_r2.fq.gz
|
3,884.04
3,859.78
|
| CRR053687 |
CRR053687_f1.fq.gz
CRR053687_r2.fq.gz
|
3,768.48
3,743.58
|
|
| Submitter | ? ?? (1337657902@qq.com) |
|---|
| Organization | Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2019-04-18 |
|---|
English
中文
Français