Experiment information
Accession CRX048581
Organism Ovis aries
Title we sequence this Shiping Gray sheep SPS14 with whole-genome resequencing
BioProject PRJCA001227
BioSample SAMC067941
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
Genomic DNA was extracted from ear tissue using the standard phenol-chloroform protocol. High-quality DNA for genome sequencing was processed to construct short-insert (500 bp) DNA libraries according to the manufacturer’s specifications. To generate 500-bp mate-paired libraries, we used the Covaris Ultrasonic Processor to cut genomic DNA into 500-bp fragments randomly, followed by the process of end repairing, adding A to the tails, purification and PCR amplification. The qualified libraries with appropriate insert size (500 bp) and concentration (> 2 nM) were sequenced using the Illumina HiSeq 2000 platform, and 100-bp paired-end reads were generated and managed using Illumina HiSeq Control Software (HCS) v3.3. WGS GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 100
Planned read length (bp) for mate 2: 100
Insert size (bp): 500
Release date2019-05-27
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR053764 2019-05-27 SPS14.sorted.bam 11,490.86
Submitter朱 强辉 (1337657902@qq.com)
OrganizationsInstitute of Zoology, Chinese Academy of Sciences
Date submitted2019-04-19