Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
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High-quality genomic DNA was extracted from leaves using a modified CTAB method20. RNase A and proteinase K were separately used to remove RNA and protein contamination. The quality and quantity of the isolated DNA were separately checked by electrophoresis on a 0.8% agarose gel and a NanoDrop D-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). A total of eleven paired-end libraries, including four types of small-insert libraries (180 bp, 260 bp, 300 bp, 500 bp) and seven large-insert libraries (2 Kb, 3 Kb, 4 Kb, 5 Kb, 6 Kb, 8 Kb, 20 Kb), were prepared following the Illumina’s instructions, and sequenced using Illumina HiSeq2000 platform by following the standard Illumina protocols (Illumina, San Diego, CA). |
WGA |
GENOMIC |
unspecified |
PAIRED
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