Experiment information
Accession CRX049642
Organism Camellia sinensis var. assamica
Title Tea tree (Camellia sinensis var. assamica) whole genome sequencing of library_1
BioProject PRJCA001416
BioSample SAMC069442
Platform Illumina HiSeq 2000
Library
Library name Construction protocol Strategy Source Selection Layout
High-quality genomic DNA was extracted from leaves using a modified CTAB method20. RNase A and proteinase K were separately used to remove RNA and protein contamination. The quality and quantity of the isolated DNA were separately checked by electrophoresis on a 0.8% agarose gel and a NanoDrop D-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). A total of eleven paired-end libraries, including four types of small-insert libraries (180 bp, 260 bp, 300 bp, 500 bp) and seven large-insert libraries (2 Kb, 3 Kb, 4 Kb, 5 Kb, 6 Kb, 8 Kb, 20 Kb), were prepared following the Illumina’s instructions, and sequenced using Illumina HiSeq2000 platform by following the standard Illumina protocols (Illumina, San Diego, CA). WGA GENOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 3000
Release date2019-05-14
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR055194 2019-05-14 T1_4_1.fastq.gz
T1_4_2.fastq.gz
15,344.77
14,737.28
SubmitterFen Zhang (zhangfen@stu.scau.edu.cn)
OrganizationsBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2019-05-06
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