Experiment information
Accession CRX049643
Organism Homo sapiens
Title OKC-18
BioProject PRJCA001417
BioSample SAMC069443
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Qualified genomic DNA from five OKC lesions and matched normal tissues were randomly fragmented by Covaris, ligated to Illumina-sequenced adapters, and selected for lengths from 150 to 200 bp. Extracted DNAs (150–200 bp) were then amplified by ligation-mediated PCR (LM-PCR), purified, and hybridized to the Agilent SureSelect Human All Exon (50M) array for enrichment. Hybridized fragments were bound to streptavidin beads, whereas nonhybridized fragments were washed out after 24 h. We then evaluated the captured LM-PCR products using an AAT1 Fragment Analyzer to estimate the magnitude of enrichment. DNA sequencing was performed on an Illumina HiSeq2500 instrument using standard protocols for a 100-bp paired-end run. Six samples were run per flowcell, guaranteeing 90% completeness at a minimum of 10× coverage. WES GENOMIC unspecified PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 300
Release date2019-05-14
Run
Run accession Run data file information
File nameFile size (MB)
CRR055508 CRR055508_f1.fastq.gz
CRR055508_r2.fastq.gz
450.28
441.2
CRR055510 CRR055510_f1.fastq.gz
CRR055510_r2.fastq.gz
1,406.45
1,380.46
SubmitterJiafei Qu (maya9090@126.com)
OrganizationPeking University School and Hospital of Stomatology
Date submitted2019-05-07