Accession | CRX049947 |
Organism | Homo sapiens |
Title | OKC-19 |
BioProject | PRJCA001417 |
BioSample | SAMC069444 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Qualified genomic DNA from five OKC lesions and matched normal tissues were randomly fragmented by Covaris, ligated to Illumina-sequenced adapters, and selected for lengths from 150 to 200 bp. Extracted DNAs (150–200 bp) were then amplified by ligation-mediated PCR (LM-PCR), purified, and hybridized to the Agilent SureSelect Human All Exon (50M) array for enrichment. Hybridized fragments were bound to streptavidin beads, whereas nonhybridized fragments were washed out after 24 h. We then evaluated the captured LM-PCR products using an AAT1 Fragment Analyzer to estimate the magnitude of enrichment. DNA sequencing was performed on an Illumina HiSeq2500 instrument using standard protocols for a 100-bp paired-end run. Six samples were run per flowcell, guaranteeing 90% completeness at a minimum of 10× coverage. |
WES |
GENOMIC |
unspecified |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 300
|
Release date | 2019-05-14 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR055511 |
CRR055511_f1.fastq.gz
CRR055511_r2.fastq.gz
|
1,474.42
1,449.1
|
CRR055512 |
CRR055512_f1.fastq.gz
CRR055512_r2.fastq.gz
|
471.2
462.46
|
|
Submitter | Jiafei Qu (maya9090@126.com) |
Organization | Peking University School and Hospital of Stomatology |
Date submitted | 2019-05-08 |