Accession | CRX050051 |
Organism | Equus asinus |
Title | RNA-seq:DS2 |
BioProject | PRJCA001418 |
BioSample | SAMC069832 |
Platform | Illumina HiSeq 2500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA from the frozen samples was extracted using the TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol. Genomic DNA contamination was first removed using RNA-free DNase I, and the RNA integrity and quality were then analyzed using a Bioanalyzer (Agilent, USA). The RNA integrity threshold was RIN ? 6.8. PolyA(+) RNA-seq libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations. The resulting cDNA was first cleaved into 300-500-bp fragments to construct libraries according to the manufacturer’s instructions, and the libraries were then sequenced using the Illumina HiSeq 2000/2500 platform |
RNA-Seq |
TRANSCRIPTOMIC |
PolyA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 150
|
Release date | 2019-06-01 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR055623 |
CRR055623_f1.fastq.gz
CRR055623_r2.fastq.gz
|
1,703.87
1,658.41
|
|
Submitter | Chen Weihuang (chenweiihuang@163.com) |
Organization | Northwest A&F University |
Date submitted | 2019-05-10 |