Experiment information
Accession CRX050544
Organism Homo sapiens
Title Wei He Lab BCG-1218-D
BioProject PRJCA001453
BioSample SAMC070643
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
We separated PBMCs cultured in RPMI-1640 medium (Gibco, CA, USA) supplemented with 10% fetal calf serum (FCS, Gibco, CA, USA), and 200 IU/mL of IL-2 in a 48-well culture plate containing immobilized BCG vaccine at 37°C in 5% CO2. After 72 h, BCG was removed. Cells were cultured until day 12-14. Stored them with RNA protection reagent in -80°C. After reverse transcription into cDNA using Total RNA, the variable region was amplified using cDNA as a template. Then they were sequenced on IIIummia MiSeq. AMPLICON TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Insert size (bp): 530
Release date2019-05-29
Run
Run accession Run data file information
File nameFile size (MB)
CRR056090 CRR056090_f1.fastq.gz
CRR056090_r2.fastq.gz
267.44
267.44
SubmitterYuxia Li (lyx_16ncmc@163.com)
OrganizationInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
Date submitted2019-05-28