Experiment information
Accession CRX050557
Organism Brassica rapa subsp. rapa
Title Drought treatment of Brassica rapa var.rapa
BioProject PRJCA001466
BioSample SAMC070676
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Bra-14days-48a-3 Total RNAs were isolated from KTRG-B48a and KTRG-B48b in 0d and 7d stages of drought treatment, and poly(A)+ RNAs were purified, and interrupted to short fragments by adding the fragmentation buffer. Next, a random hexamer-primer was used to synthesize the first-strand cDNA using these short fragments as templates, then the second-strand cDNA was synthesized using RNaseH and DNA polymerase I. Fragments were retrieved and connected with sequencing adapters. Next, fragments were selected using agarose gel electrophoresis separation, and then suitable fragments were amplified as templates. The library was paired-end -150 bp sequenced using an Illumina HiSeq 4000 RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 150
Release date2019-06-04
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR056266 2019-06-04 FCHCNJJALXX_L5_WHBRAnxvTAAIRAAPEI-154_1.fq.gz
FCHCNJJALXX_L5_WHBRAnxvTAAIRAAPEI-154_2.fq.gz
1,490.73
1,642.56
Submitter丹妮 杨 (yangdanni@mail.kib.ac.cn)
OrganizationsKIB of UCAS
Date submitted2019-05-28