Experiment information
Accession CRX051272
Organism Macaca fascicularis
Title VPA-treated2-1
BioProject PRJCA001509
BioSample SAMC071531
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from the PFC of VPA-exposed and age-matched control monkeys by Trizol reagent following the manufacturers instruction (Invitrogen). The RNA quality was examined by Bioanalyzer 2109 (Agilent) and the RIN (RNA integrity number) over8 was qualified for cDNA library construction. First-strand cDNA was synthesized with a SuperScript III First Strand cDNA synthesis kit (Invitrogen) according to the manufacturer's protocol, followed by second strand cDNA synthesis using RNase H and DNA polymerase I (Invitrogen). Sequencing was performed on a single lane of an Illumina HiSeq 4000 to produce 150 bp paired-end reads. RNA-Seq TRANSCRIPTOMIC cDNA PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-06-25
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR056882 2019-06-25 M2-1_raw_data_1.fastq.gz
M2-1_raw_data_2.fastq.gz
1,939.66
2,212.36
SubmitterQi qi Wang (wqq@genetics.ac.cn)
OrganizationsInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
Date submitted2019-06-19