Accession | CRX051273 |
Organism | Macaca fascicularis |
Title | VPA-treated2-2 |
BioProject | PRJCA001509 |
BioSample | SAMC071532 |
Platform | Illumina HiSeq 4000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted from the PFC of VPA-exposed and age-matched control monkeys by Trizol reagent following the manufacturers instruction (Invitrogen). The RNA quality was examined by Bioanalyzer 2110 (Agilent) and the RIN (RNA integrity number) over8 was qualified for cDNA library construction. First-strand cDNA was synthesized with a SuperScript III First Strand cDNA synthesis kit (Invitrogen) according to the manufacturer's protocol, followed by second strand cDNA synthesis using RNase H and DNA polymerase I (Invitrogen). Sequencing was performed on a single lane of an Illumina HiSeq 4000 to produce 150 bp paired-end reads. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2019-06-25 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR056883 |
CRR056883_f1.fastq.gz
CRR056883_r2.fastq.gz
|
2,202.95
2,456.24
|
|
Submitter | Qi qi Wang (wqq@genetics.ac.cn) |
Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
Date submitted | 2019-06-19 |