Accession | CRX051595 |
Organism | Homo sapiens |
Title | Human E -48h Fn infected |
BioProject | PRJCA001517 |
BioSample | SAMC071809 |
Platform | BGISEQ-500 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was isolated, evaluated the quality, reverse-transcribed to Cdna. 1)mRNA enrichment: Oligo dT Selection or rRNA depletion;2)RNA fragment and reverse transcription: Fragment the RNA and reverse transcription to double-strand cDNA (dscDNA) by N6 random primer;3)End repair, add A tailing and adaptor ligation:The synthesized cDNA was subjected to end-repair and then was 3’ adenylated. Adaptors were ligated to the ends of these 3’ adenylated cDNA fragments;4)PCR amplification:The ligation products were purified and many rounds of PCR amplification were performed to enrich the purified cDNA template using PCR primer;5)Denature and cyclization:Denature the PCR product by heat and the single strand DNA is cyclized by splint oligo and DNA ligase;6) Sequencing on BGISEQ-500 platform. |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
SINGLE
|
|
Processing |
Planned read length (bp): 50
|
Release date | 2019-09-12 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR057233 |
CRR057233.fq.gz
|
1,015.39
|
|
Submitter | Wenyan Kang (943818034@qq.com) |
Organization | Shandong University |
Date submitted | 2019-06-25 |