| Accession | CRX052353 |
| Organism | Microbiota |
| Title | 16S amplicon sequencing of plant phyllosphere microbiome sampleMybPhyl2R05 |
| BioProject | PRJCA001267 |
| BioSample | SAMC072588 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
DNA for each sample was extracted with FastDNA SPIN Kit (MP Biomedicals), then V5-V7(799F-1193R) of 16S rDNA was amplified by two-step PCR. Finally, the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode. |
AMPLICON |
METAGENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 250
Planned read length (bp) for mate 2: 250
Insert size (bp): 450
|
| Release date | 2019-07-05 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR058067 |
CRR058067_f1.fq.gz
CRR058067_r2.fq.gz
|
14.58
21.8
|
|
| Submitter | Yong-Xin Liu (woodcorpse@163.com) |
|---|
| Organization | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
|---|
| Date submitted | 2019-07-05 |
|---|
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