Experiment information
Accession CRX052371
Organism Gallus gallus
Title E17_2
BioProject PRJCA001556
BioSample SAMC072606
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Approximately 10 ?g of total RNA were used to deplete ribosomal RNA according to the manuscript of the Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). Following purification, the poly(A)- or poly(A)+ RNA fraction was fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insect size for the paired-end libraries was 300 bp (±50 bp). RNA-Seq TRANSCRIPTOMIC RT-PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-11-24
Run
Run accession Run data file information
File nameFile size (MB)
CRR058085 CRR058085_f1.fq.gz
CRR058085_r2.fq.gz
2,500.97
2,658.52
Submitterjie liu (liujie84130@163.com)
OrganizationPoultry Institute, Shandong Academy of Agricultural Sciences
Date submitted2019-07-05