Accession | CRX052374 |
Organism | Gallus gallus |
Title | D1_3 |
BioProject | PRJCA001556 |
BioSample | SAMC072609 |
Platform | Illumina HiSeq 4000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Approximately 10 ?g of total RNA were used to deplete ribosomal RNA according to the manuscript of the Epicentre Ribo-Zero Gold Kit (Illumina, San Diego, USA). Following purification, the poly(A)- or poly(A)+ RNA fraction was fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNA-Seq sample preparation kit (Illumina, San Diego, USA), the average insect size for the paired-end libraries was 300 bp (±50 bp). |
RNA-Seq |
TRANSCRIPTOMIC |
RT-PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2019-11-24 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR058088 |
CRR058088_f1.fq.gz
CRR058088_r2.fq.gz
|
2,779
2,920.15
|
|
Submitter | jie liu (liujie84130@163.com) |
Organization | Poultry Institute, Shandong Academy of Agricultural Sciences |
Date submitted | 2019-07-05 |