Experiment information
Accession CRX052852
Organism African swine fever virus
Title nanopore-ASFV
BioProject PRJCA001561
BioSample SAMC072713
Platform GridION
Library
Library name Construction protocol Strategy Source Selection Layout
After the nucleic acid sample quality inspection was qualified, the genomic DNA was randomly interrupted by Megaruptor (Diagenode), the large fragment DNA was enriched and purified by magnetic beads. Both ends of the fragments were repaired and added dA tail. After purification, the SQK-LSK108 ligation kit (Oxford Nanopore Technologies) was used to perform the ligation reaction, and finally used Qubit to accurately quantify the constructed DNA library. The DNA library of the specified concentration and volume was added to a flow cell, and the flow cell was transferred to a PromethION sequencer for real-time single molecule sequencing. WGS METAGENOMIC unspecified FRAGMENT
Processing
Release date2019-09-01
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR058564 2019-09-01 ont.fastq.gz 58,928.28
SubmitterLijia Jia (jialj@wh.iov.cn)
OrganizationsWuhan institute of virology
Date submitted2019-07-09
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