Experiment information
Accession CRX053990
Organism Homo sapiens
Title MBNL1 RNaseR rep1
BioProject PRJCA001600
BioSample SAMC079925
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was isolated by TRIZOL (Invitrogen, Carlsbad, CA), the quality and quantity of RNA were assessed with NanoDrop, Qubit and BioAnalyzer 2100 (Agilent). A RiboMinus kit (KAPA, USA) was used for ribosomal RNA depletion. Then, total RNA was divided into three equal parts after depletion of ribosomal RNA to construct a riboMinus and two riboMinus/RNase R treated replicates libraries. RNase R was purchased from Epicentre (Madison, WI) to digest linear transcripts. rRNA-depleted RNA was incubated at 37 °C with 10 U/?g RNase R in 16 ?l reaction. RNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 400
Release date2019-07-27
Run
Run accession Run data file information
File nameFile size (MB)
CRR060060 CRR060060_f1.fastq.gz
CRR060060_r2.fastq.gz
3,634.61
3,651.98
SubmitterJinyang Zhang (zhangjinyang@biols.ac.cn)
OrganizationBeijing Institutes of Life Sciences
Date submitted2019-07-27