| Accession | CRX053991 |
| Organism | Homo sapiens |
| Title | MBNL1 RNaseR rep2 |
| BioProject | PRJCA001600 |
| BioSample | SAMC079926 |
| Platform | Illumina HiSeq 2500 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was isolated by TRIZOL (Invitrogen, Carlsbad, CA), the quality and quantity of RNA were assessed with NanoDrop, Qubit and BioAnalyzer 2105 (Agilent). A RiboMinus kit (KAPA, USA) was used for ribosomal RNA depletion. Then, total RNA was divided i three equal parts after depletion of ribosomal RNA to construct a riboMinus and two riboMinus/RNase R treated replicates libraries. RNase R was purchased from Epicentre (Madison, WI) to digest linear transcripts. rRNA-depleted RNA was incubated at 37 °C with 10 U/?g RNase R in 16 ?l reaction.. s |
RNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 101
Planned read length (bp) for mate 2: 101
Insert size (bp): 400
|
| Release date | 2019-07-27 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR060061 |
CRR060061_f1.fastq.gz
CRR060061_r2.fastq.gz
|
2,599.99
2,615.98
|
|
| Submitter | Jinyang Zhang (zhangjinyang@biols.ac.cn) |
|---|
| Organization | Beijing Institutes of Life Sciences |
|---|
| Date submitted | 2019-07-27 |
|---|
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