Experiment information
Accession CRX060771
Organism Homo sapiens
Title Diluted_HD753_Mix1
BioProject PRJCA001652
BioSample SAMC090970
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
HD753 was mixed with Hapmap Normal cell line NA18536.Diluted DNA mixtures were further sheared using a focused-ultrasonicator with a target size around 170 bp to mimic the size distribution of cfDNA. 33 ng sheared reference sample was used for end repair and A-tailing, followed by ligation of SinoDuplex adapters and amplification using KAPA HiFi HotStart ReadyMix with unique dual indexes.Targeted capture was performed with customized panel according to IDT xGen Hybridization and Wash Kit protocol. Targeted-Capture GENOMIC Hybrid Selection PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 180
Release date2019-08-28
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR071400 2019-08-28 R18059478-CA18092816-RNDHD753M1P27_combined_R1.fastq.gz
R18059478-CA18092816-RNDHD753M1P27_combined_R2.fastq.gz
13,463.87
14,478
SubmitterBinggding Huang (huangbingding@sztu.edu.cn)
OrganizationsShenzhen Technology University
Date submitted2019-08-27
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