Experiment information
Accession CRX061282
Organism Homo sapiens
Title RKO-ph7.4-2
BioProject PRJCA001709
BioSample SAMC097230
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
The total RNA in RKO cells cultured in pH 7.4 and pH 6.8 conditions was isolated with TRIzol reagent(Life Technologies). The RNA integrity was measured at an Agilent Bioanalyzer 2100 instrument (Agilent Technologies, Santa Clara, CA,USA). The qualified total RNA was further purified (RNeasy Mini Kit, Qiagen, Hilden, Germany). RNA-Seq TRANSCRIPTOMIC RANDOM PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 400
Release date2019-08-31
Run
Run Accession Release date Run Data File Information
File nameFile size (MB)
CRR071911 2019-08-31 RKO-pH-4-2_RRAS03359-V_1.fq.gz
RKO-pH-4-2_RRAS03359-V_2.fq.gz
4,154.14
4,343.81
SubmitterPeishan Hu (xiaopei296@sina.com)
OrganizationsSun Yat-sen University Cancer Center
Date submitted2019-08-30
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Experiments(5)