Experiment information
Accession CRX074562
Organism Gallus gallus
Title C_R4
BioProject PRJCA001898
BioSample SAMC116228
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Firstly, ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNAdepleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ncRNA-Seq TRANSCRIPTOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-11-23
Run
Run accession Run data file information
File nameFile size (MB)
CRR087491 CRR087491_f1.fq.gz
CRR087491_r2.fq.gz
3,536
4,188.52
SubmitterXiaodong Tan (tanxiaodong08@163.com)
OrganizationInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences
Date submitted2019-11-22