| Accession | CRX074564 |
| Organism | Gallus gallus |
| Title | FL_R2 |
| BioProject | PRJCA001898 |
| BioSample | SAMC116230 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Firstly, ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNAdepleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations |
ncRNA-Seq |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| Release date | 2019-11-23 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR087493 |
CRR087493_f1.fq.gz
CRR087493_r2.fq.gz
|
3,738.77
4,200.5
|
|
| Submitter | Xiaodong Tan (tanxiaodong08@163.com) |
|---|
| Organization | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
|---|
| Date submitted | 2019-11-22 |
|---|
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