Accession | CRX074566 |
Organism | Gallus gallus |
Title | C_M1 |
BioProject | PRJCA001898 |
BioSample | SAMC116232 |
Platform | Illumina HiSeq X Ten |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
A total amount of 5.2 microgram genomic DNA spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer’s instructions.Then these DNA fragments
were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit
(Zymo Research), before the resulting single-strand DNA fragments were PCR
amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X) |
Bisulfite-Seq |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
Release date | 2019-11-23 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR087495 |
CRR087495_f1.fq.gz
CRR087495_r2.fq.gz
|
7,999.52
7,765.05
|
|
Submitter | Xiaodong Tan (tanxiaodong08@163.com) |
Organization | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
Date submitted | 2019-11-22 |