Experiment information
Accession CRX074566
Organism Gallus gallus
Title C_M1
BioProject PRJCA001898
BioSample SAMC116232
Platform Illumina HiSeq X Ten
Library
Library name Construction protocol Strategy Source Selection Layout
A total amount of 5.2 microgram genomic DNA spiked with 26 ng lambda DNA were fragmented by sonication to 200-300bp with Covaris S220, followed by end repair and adenylation. Cytosine-methylated barcodes were ligated to sonicated DNA as per manufacturer’s instructions.Then these DNA fragments were treated twice with bisulfite using EZ DNA Methylation-GoldTM Kit (Zymo Research), before the resulting single-strand DNA fragments were PCR amplificated using KAPA HiFi HotStart Uracil + ReadyMix (2X) Bisulfite-Seq GENOMIC PCR PAIRED
Processing Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
Release date2019-11-23
Run
Run accession Run data file information
File nameFile size (MB)
CRR087495 CRR087495_f1.fq.gz
CRR087495_r2.fq.gz
7,999.52
7,765.05
SubmitterXiaodong Tan (tanxiaodong08@163.com)
OrganizationInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences
Date submitted2019-11-22