Experiment information
Accession CRX075078
Organism human gut metagenome
Title CAD_gut_171_16S
BioProject PRJCA001924
BioSample SAMC116783
Platform Illumina MiSeq
Library
Library name Construction protocol Strategy Source Selection Layout
The fresh fecal samples were obtained from 190 individuals and all of the samples were stored at -80 degree centigrade until DNA extraction. A total of 200 mg of each fecal sample was used for DNA extraction according to the manufacturer’s instructions. The KAPA was used of amplify the 16S rRNA (V3-V4) gene tags. Each DNA sample of the bacterial 16S rRNA gene was generated by amplified with primer 341F and 805R. The products were submitted to the next-generation sequencing laboratory of Novogene Bioinformatics Institute, Beijing, China. OTHER METAGENOMIC other PAIRED
Processing Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Insert size (bp): 500
Release date2019-12-11
Run
Run accession Run data file information
File nameFile size (MB)
CRR088095 CRR088095_f1.fastq.gz
CRR088095_r2.fastq.gz
5.19
5.33
Submitterzilong he (koheallen@163.com)
OrganizationBeijing Institute of Genomics, Chinese Academy of Sciences
Date submitted2019-11-28
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