| Accession | CRX082710 |
| Organism | Mus musculus |
| Title | coverage_ESCs |
| BioProject | PRJCA002101 |
| BioSample | SAMC127076 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Inserted sites were isolated using splinkerette PCR and high throughput sequencing. The second round of the nested PCR primers was used as barcodes to filter reads. We then extracted the sequences contained "TTAA" using cutadapt, and then aligned them to mouse reference genome from ensembl release 97 using bwa aligner. PCR duplications in alignments were removed using gatk, and cleaned alignments were used to count reads with the window of 100,000 bp using deeptools2. |
AMPLICON |
GENOMIC |
PCR |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 350
|
| Release date | 2020-01-08 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR106355 |
CRR106355_f1.fastq.gz
CRR106355_r2.fastq.gz
|
2,879.77
2,919.47
|
|
| Submitter | Junjie Mao (learnbetter@hotmail.com) |
|---|
| Organization | Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2020-01-07 |
|---|
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