Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
osmotic3 |
P. trichocarpa plants were grown in a greenhouse under a 16 h light/8 h dark photoperiod, with light provided by cool white fluorescent lights (250 μmol m-2 s-1 PPFD). For stress treatments, 12 plants obtained from a single genotype were used for chilling stress (three plants; 4°C, 6 h, 250 μmol m-2 s-1 PPFD),for heat stress (three plants; 42°C, 6 h, 250 μmol m-2 s-1 PPFD), three were exposed to 150 mM NaCl, 30% polyethylene glycol (PEG) 6000 for 6 h, and three were exposed to a drought treatment (25°C, 250 μmol m-2 s-1 PPFD, soil moisture contents 15-20%). Leaves were collected from P. trichocarpa for RNA extraction with different treatment (three biological replicates per treatment). |
RNA-Seq |
TRANSCRIPTOMIC |
RANDOM |
PAIRED
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