| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Whole-exome sequencing was performed by a sequencing-by-ligation method. Briefly, fragmented genomic DNA was generated by Covaris system with length between 200-400bp. After ligation and PCR amplification, the whole exome is enriched by Exome 59M kit (BGI). Then, a second round PCR amplification is performed. The resulted DNA fragments are prepared for Complete Genomics Black Bird Sequencer, and 30–35 base paired-end reads are finally obtained. |
WXS |
GENOMIC |
PCR |
PAIRED
|
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