Accession | CRX021692 |
Organism | Homo sapiens |
Title | ILMN40T |
BioProject | PRJCA000610 |
BioSample | SAMC018567 |
Platform | Illumina HiSeq 2000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Human genomic DNA was extracted from frozen tumor or matched colon tissue with QIAampDNA Mini kits (Qiagen). Covaris system is used to obtain the fragmented genomic DNA between 150 to 200bp. Adaptors were ligated to both ends of the fragments and adaptor-ligated templates were then purified using AgencourtAMPure SPRI beads. Whole-exome enrichment was performed by the SureSelect Human All Exon 51M kit (Agilent). Captured DNA libraries are sequenced by HiSeq 2000 Genome Analyzer (Illumina). |
WXS |
GENOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 90
Planned read length (bp) for mate 2: 90
Insert size (bp): 170
|
Release date | 2018-05-29 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR023936 |
CRR023936.bam
|
12,263.2
|
|
Submitter | Zhe Liu (liuzhebiosci@163.com) |
Organization | Anzhen Hospital |
Date submitted | 2017-12-01 |