Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
3_18_CD14 |
Library preparation for LncRNA sequencing. A total amount of 3 ?g RNA per sample was used as initial material for the RNA sample preparations. Ribosomal RNA was removed using Epicentre Ribo-ZeroTM Gold Kits(Human/Mouse/Rat) (Epicentre, USA). Subsequently, the sequencing libraries were generated following manufacturer recommendations with varied index label by NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA). Libraries were sequenced on Illumina HiSeq X Ten (Hiseq x Ten Reagent Kit v2.5). |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
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