Experiment information
Accession CRX030659
Organism Mus musculus
Title ESC_early
BioProject PRJCA000997
BioSample SAMC046985
Platform Illumina HiSeq 4000
Library
Library name Construction protocol Strategy Source Selection Layout
Reduced representation bisulfite sequencing (RRBS) for a global DNA methylation analysis of wild type (WT) or the differentially methylated region knocking out (DMR-KO) haploid ESCs androgenetic haploid ESCs (ahESC), parthenogenetic haploid ESCs (phESCs), diploid ESCs, embryos and brain tissue. Compared to wild type (WT) embryo, which was produced by normal zygote deriving from normal sperm and oocyte, the bimaternal (BM) embryo was produced through injecting a phESC into an MII oocyte and the bipaternal (BP) embryo was produced through injecting injecting an ahESC and a sperm into an enucleated oocyte. Bisulfite-Seq GENOMIC Reduced Representation PAIRED
Processing Planned read length (bp) for mate 1: 50
Planned read length (bp) for mate 2: 50
Insert size (bp): 200
Release date2018-10-17
Run
Run accession Run data file information
File nameFile size (MB)
CRR034175 CRR034175_f1.fq.gz
CRR034175_r2.fq.gz
1,878.19
2,029.91
CRR034176 CRR034176_f1.fq.gz
CRR034176_r2.fq.gz
1,277.75
1,370.1
SubmitterGuihai Feng (fenggh@aliyun.com)
OrganizationInstitute of Zoology, Chinese Academy of Sciences
Date submitted2018-10-02