1. Home
  2. Browse
  3. CRA001131
  4. CRX030280
Experiment information
Accession CRX030280
Organism Mus musculus
Title mESC_bulk_Not1
BioProject PRJCA001042
BioSample SAMC046630
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from approximately one million mESCs using a miRNeasy Micro kit (QIAGEN). The libraries were constructed following the manufacturer's protocol using NEBNext kits (E7490&E7530), before PCR enrichment, the DNA was digested by Not1 at 37 degree Celsius for 2h RNA-Seq TRANSCRIPTOMIC PolyA SINGLE
Processing Planned read length (bp): 50
Release date2018-09-30
Run
Run accession Run data file information
File nameFile size (MB)
CRR033726 CRR033726.fastq.gz 764.25
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationZhejiang University
Date submitted2018-09-23
Related experiments
Experiments(76)