Experiment information
Accession CRX030282
Organism Mus musculus
Title Z4_1ng_small_2
BioProject PRJCA001042
BioSample SAMC046628
Platform Illumina HiSeq 2500
Library
Library name Construction protocol Strategy Source Selection Layout
Total RNA was extracted from approximately one million mESCs using an RNeasy Plus Mini kit (QIAGEN), 1ng diluted total RNA was mixed with with 100 ng small carrier RNA. The small RNA sequencing library was constructed following the manufacturer's protocol using a NEBNext kit (NEB #E7300). After PCR amplification (15 cycles), the cDNA fragment from the RNA carrier was removed by Not1 digestion. After Not1 digestion, the library DNA was purified for deep sequencing. RNA-Seq TRANSCRIPTOMIC other SINGLE
Processing Planned read length (bp): 50
Release date2018-09-30
Run
Run accession Run data file information
File nameFile size (MB)
CRR033724 CRR033724.fastq.gz 86.74
SubmitterZhengyun Xiao (xiaozhengyun1993@126.com)
OrganizationZhejiang University
Date submitted2018-09-23