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Experiment information

Accession

CRX030286

Organism

Title

mESC_smart_1ng_2

BioProject

BioSample

Platform

Illumina HiSeq 2500

Library

Library name	Construction protocol	Strategy	Source	Selection	Layout
	cDNA from 1 ng of total mESC RNA was produced following the manufacturer's protocol using the Smart-Seq2 v4 Ultra Low Input RNA kit (Clontech) for single cells. Then, 2 ng of cDNA was sheared into 150- to 350-bp fragments by sonication, and a NEBNext DNA library preparation kit (E7645) was used to construct the sequencing library.	RNA-Seq	TRANSCRIPTOMIC	PolyA	SINGLE

Processing

Planned read length (bp): 50

Release date

2018-09-30

Run

Run accession	Run data file information
Run accession	File name	File size (MB)
CRR033720	CRR033720.fastq.gz	461.55

Submitter

Zhengyun Xiao (xiaozhengyun1993@126.com)

Organization

Zhejiang University

Date submitted

2018-09-23

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