| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
| 3-6kb |
Following isolation, purification and determination were performed using the method mentioned in the SGS process, and the total RNA of the combined sample was reversed-transcribed into first-strand cDNA using a SMARTer PCR cDNA Synthesis Kit (Clontech Laboratories, USA). The cDNA was then amplified by large-scale PCR for 10 cycles, column-purified, and then size-selected into cDNA fractions in sizes of 1-2 kb, 2-3 kb, and 3-6 kb using a BluePippin System (Sage Science, USA). Two SMRT cells for the 1-2 kb fractions, two for the 2-3 kb fractions, and one for the 3-6 kb fractions were run on the PacBio RS II platform for sequencing. |
FL-cDNA |
TRANSCRIPTOMIC |
RT-PCR |
SINGLE
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