Accession | CRX032484 |
Organism | Mus musculus |
Title | ESC2 |
BioProject | PRJCA001125 |
BioSample | SAMC048027 |
Platform | Illumina HiSeq 4000 |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted from cultured cells by TRIzol reagent. For RNA-seq library construction, the PolyA+ tailed RNA purification was performed for each sample using a Dynabead mRNA purification kit. The cDNA library was generated with a KAPA stranded mRNA-Seq Kit protocol. Sequencing was performed on an Illumina HiSeq 4000 platform, with 150 bp paired-end-sequencing reactions. |
RNA-Seq |
GENOMIC |
PolyA |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 300
|
Release date | 2019-01-10 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR037077 |
CRR037077_f1.fastq.gz
CRR037077_r2.fastq.gz
|
2,824.06
2,939
|
|
Submitter | Guihai Feng (fenggh@aliyun.com) |
Organization | Institute of Zoology, Chinese Academy of Sciences |
Date submitted | 2018-11-17 |