| Accession | CRX032485 |
| Organism | Mus musculus |
| Title | MEF |
| BioProject | PRJCA001125 |
| BioSample | SAMC048028 |
| Platform | Illumina HiSeq 4000 |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
Total RNA was extracted from cultured cells by TRIzol reagent. For RNA-seq library construction, the PolyA+ tailed RNA purification was performed for each sample using a Dynabead mRNA purification kit. The cDNA library was generated with a KAPA stranded mRNA-Seq Kit protocol. Sequencing was performed on an Illumina HiSeq 4000 platform, with 150 bp paired-end-sequencing reactions. |
RNA-Seq |
GENOMIC |
PolyA |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 125
Planned read length (bp) for mate 2: 125
Insert size (bp): 300
|
| Release date | 2019-01-10 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR037078 |
CRR037078_f1.fastq.gz
CRR037078_r2.fastq.gz
|
2,667.46
2,659.36
|
|
| Submitter | Guihai Feng (fenggh@aliyun.com) |
|---|
| Organization | Institute of Zoology, Chinese Academy of Sciences |
|---|
| Date submitted | 2018-11-17 |
|---|
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