Accession | CRX040236 |
Organism | Homo sapiens |
Title | A survey of the transcriptional landscape in esophageal squamous cells with long read single molecule real-time sequencing |
BioProject | PRJCA001228 |
BioSample | SAMC055205 |
Platform | PacBio Sequel |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
The libraries was prepared according to the Isoform Sequencing protocol (Iso-Seq) as described by Pacific Biosciences (PN100-092-800-03). Firstly the cDNA was synthesized by total mRNAs for each sample using the Clontech SMARTer PCR cDNA Synthesis Kit. To increase the sequencing yield of long transcripts, the Blue Pippin Size Selection System protocol was used to select the >4kb fragments for each sample after the PCR amplification of the cDNAs. Then the equi-molar mixture of long cDNA fragments (>4kb) and normal cDNAs were used subsequently for SMRT sequencing. |
Synthetic-long-Read |
TRANSCRIPTOMIC |
PCR |
SINGLE
|
|
Processing |
|
Release date | 2019-07-10 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR044978 |
CRR044978.tar.gz
|
13,432.13
|
CRR044979 |
CRR044979.tar.gz
|
13,911.14
|
|
Submitter | Yunmei Chen (chenyunmei@novogene.com) |
Organization | Novogene |
Date submitted | 2019-01-19 |