Accession | CRX050552 |
Organism | Homo sapiens |
Title | Wei He Lab Control-1217-G |
BioProject | PRJCA001453 |
BioSample | SAMC070646 |
Platform | Illumina MiSeq |
Library |
Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
We separated PBMCs cultured in RPMI-1640 medium (Gibco, CA, USA) supplemented with 10% fetal calf serum (FCS, Gibco, CA, USA), and 200 IU/mL of IL-2 in a 48-well culture plate containing immobilized BCG vaccine at 37°C in 5% CO2. After 72 h, BCG was removed. Cells were cultured until day 12-14. Stored them with RNA protection reagent in -80°C. After reverse transcription into cDNA using Total RNA, the variable region was amplified using cDNA as a template. Then they were sequenced on IIIummia MiSeq. |
AMPLICON |
TRANSCRIPTOMIC |
PCR |
PAIRED
|
|
Processing |
Planned read length (bp) for mate 1: 300
Planned read length (bp) for mate 2: 300
Insert size (bp): 530
|
Release date | 2019-05-29 |
Run |
Run accession |
Run data file information |
File name | File size (MB) |
CRR056098 |
CRR056098_f1.fastq.gz
CRR056098_r2.fastq.gz
|
287.85
287.85
|
|
Submitter | Yuxia Li (lyx_16ncmc@163.com) |
Organization | Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College |
Date submitted | 2019-05-28 |