| Accession | CRX051220 |
| Organism | Eriobotrya japonica |
| Title | The clean reads in the next generation sequencing of loquat |
| BioProject | PRJCA001507 |
| BioSample | SAMC071464 |
| Platform | Illumina HiSeq X Ten |
| Library |
| Library name |
Construction protocol |
Strategy |
Source |
Selection |
Layout |
|
High-quality genomic DNA was randomly interrupted by ultrasound. DNA fragments ranging from 200~500 bp were recovered by electrophoresis. T4 DNA Polymerase, Klenow DNA Polymerase and T4 PNK were used to repair cohesive ends to blunt ends. The 3′ end of DNA fragment was amended including an “A” base and ligated to adaptors, including a 5′ end T base. All fragments were recovered by electrophoresis and then paired-end sequenced by an Illumina HiSeq X Ten (San Diego, CA, USA). The adaptors and low-quality reads with more than 20% bases of quality value ≤10 in raw sequence data in FASTQ format were filtered by Trimmomatic. Only clean reads were used in the subsequent analysis. |
WGA |
GENOMIC |
unspecified |
PAIRED
|
|
| Processing |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 500
|
| Release date | 2019-06-24 |
|---|
| Run |
| Run accession |
Run data file information |
| File name | File size (MB) |
|---|
| CRR056810 |
CRR056810_f1.fq.gz
CRR056810_r2.fq.gz
|
13,241.23
14,104.35
|
|
| Submitter | Shuang Jiang (271339616@qq.com) |
|---|
| Organization | Shanghai Academy of Agricultural Sciences |
|---|
| Date submitted | 2019-06-17 |
|---|
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