| 实验编号 | CRX051263 |
| 物种名称 | Macaca fascicularis |
| 标题 | Control1-1 |
| 项目编号 | PRJCA001509 |
| 样本编号 | SAMC071522 |
| 测序平台 | Illumina HiSeq 4000 |
| 建库信息 |
| 文库名称 |
文库构建方法 |
建库策略 |
样品来源 |
实验选择 |
文库布局 |
|
Total RNA was extracted from the PFC of VPA-exposed and age-matched control monkeys by Trizol reagent following the manufacturers instruction (Invitrogen). The RNA quality was examined by Bioanalyzer 2100 (Agilent) and the RIN (RNA integrity number) over 6.8 was qualified for cDNA library construction. First-strand cDNA was synthesized with a SuperScript III First Strand cDNA synthesis kit (Invitrogen) according to the manufacturer's protocol, followed by second strand cDNA synthesis using RNase H and DNA polymerase I (Invitrogen). Sequencing was performed on a single lane of an Illumina HiSeq 4000 to produce 150 bp paired-end reads. |
RNA-Seq |
TRANSCRIPTOMIC |
cDNA |
PAIRED
|
|
| 处理信息 |
Planned read length (bp) for mate 1: 150
Planned read length (bp) for mate 2: 150
Insert size (bp): 300
|
| 发布日期 | 2019-06-25 |
|---|
| 测序反应 |
| Run编号 |
Run序列文件信息 |
| File name | File size (MB) |
|---|
| CRR056873 |
CRR056873_f1.fq.gz
CRR056873_r2.fq.gz
|
2,070.83
2,441.31
|
|
| 提交者 | Qi qi Wang (wqq@genetics.ac.cn) |
|---|
| 所属单位 | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences |
|---|
| 提交日期 | 2019-06-19 |
|---|
English
中文
Français